In This Issue
Frontiers of Engineering
December 1, 2008 Volume 38 Issue 4
Winter 2008 issue of The Bridge on Frontiers of Engineering

Recent Development in Needle-Free Drug Delivery

Monday, December 1, 2008

Author: Samir Mitragotri

Macromolecular drugs can be delivered with painless, patient-friendly alternatives to injections.

Delivering medicines to patients in a safe, effective, and compliant way can be a major challenge (Langer, 2003). Pills and injections are the most common modalities for administering drugs. Although pills can only deliver small molecules, they are generally accepted as a convenient mode of drug delivery (Morishita and Peppas, 2006). Macromolecular drugs such as peptides and proteins, which cannot be taken orally, must be administered by injection. For some drugs, however, systemic administration to healthy tissues can be toxic, regardless of how they are administered. These drugs are only effective if they act directly on specific diseased tissues (Vasir and Labhasetwar, 2005).

The ability of drugs to reach target tissues from the point of administration via pills or injections is limited by the body’s multiple barriers, including enzymatic degradation in the stomach, absorption across the intestinal epithelium, hepatic clearance, and accumulation in non-targeted tissues. These barriers have a range of lengths (from the tissue to the organelle level) and time scales.

Collectively, these conditions have made the conversion of potent biomolecules into medical therapies very challenging. The field of drug delivery has grown in response to these challenges and is now a significant component of the overall drug-development process.

In the past several decades, tremendous progress has been made toward the development of safe, effective, and convenient means of drug administration. Advances have been possible, at least in part, because of our improved understanding of the human body. This article focuses on some key developments in the field of drug delivery, especially those that deal with the development of painless, patient-friendly alternatives to injections for the delivery of macromolecules (Figure 1).

Figure 1 modes of needle-free drug delivery

FIGURE 1 Various modes of needle-free drug delivery. Source: Adapted from Mitragotri, 2005.

The Need for Better Methods of Drug Delivery
Needles and syringes are the most common method of administering macromolecular drugs; an estimated 12 billion injections are given annually worldwide (Kermode, 2004). Despite their common use, needles have several limitations, including needle phobia (Nir et al., 2003) and accidental needle sticks (Rosenstock, 2000). In addition, concerns have arisen about the unsafe use of needles, as exemplified by the overwhelming number of HIV, hepatitis C, and hepatitis B infections that are thought to originate each year from the re-use of needles and syringes (Kane et al., 1999).

Noncompliance with medical treatment regimes is also a significant issue. It has been estimated that most patients do not adhere to prescribed dosing regimens, even in developed countries. Noncompliance is linked to several factors, including pain, needle phobia, and forgetfulness, and can result in serious medical complications. In fact, noncompliance is a leading cause of hospitalizations when the carefully designed drug concentration profile is altered in a way that becomes harmful to the patient.

Typically, the blood concentration levels of both injectable and oral drugs that are administered repeatedly vary, depending on the schedule of their administration and the speed at which they are absorbed and distributed by the body. Deviations from the therapeutic range of blood concentrations cause undesirable effects. For these reasons, it is important that drug developers, in addition to considering the efficacy and safety of a drug, must also carefully consider how a drug-delivery system may affect patient compliance.

The limitations of conventional methods of drug delivery can potentially be overcome by needle-free delivery of drugs through the skin or mucosal surfaces of the mouth, nose, or lungs (Varmus et al., 2003). Although these represent viable alternatives to needle-based methods, these surfaces also present significant barriers to drug entry into the body, and breaching them in a safe, effective way is a major goal of drug-delivery research. This article, provides a brief review of past efforts, a description of the current status, and prospects for the future, with an emphasis on transdermal and oral drug delivery.

Transdermal Drug Delivery
Skin, the largest human organ, provides a painless, compliant interface for systemic drug administration (Zaffaroni, 1991). However, because skin evolved to impede the flux of toxins into the body, it naturally has low permeability to the movement of foreign molecules (Wertz and Downing, 1989). A unique, hierarchical structure of lipid-rich matrix with embedded corneocytes in the stratum corneum (the upper strata [15 µm] of skin), is responsible for this barrier (Wertz and Downing, 1989).

Corneocytes, cross-linked keratin fibers (about 0.2–0.4 microns thick and about 40 microns wide) held together by corneodesmosomes, provide structural stability to the stratum corneum. Lipids, which provide the primary barrier function in the stratum corneum, consist of several components; the primary constituents are ceramides, cholesterol, and fatty acids. The layer of lipids immediately adjacent to the corneocytes is covalently bound to them and plays an important role in maintaining the barrier function. The stratum corneum is continuously desquamated, with a renewal period of about one week, and is actively repaired by the secretion of lamellar bodies following the disruption of the barrier properties or other environmental insults (Prausnitz et al., 2004).

Transdermal drug delivery involves placing a drug on the skin in the form of a patch, cream, or lotion wherein the drug permeates across the skin and enters the bloodstream. Key advantages of transdermal delivery include the easy accessibility of skin, which encourages patient compliance, avoidance of the gastrointestinal tract, and sustained release over extended periods of time (Prausnitz et al., 2004).

A number of drugs, including scopolamine, nitroglycerin, nicotine, clonidine, fentanyl, estradiol, testosterone, lidocaine, and oxybutinin, are routinely delivered transdermally by skin patches (Prausnitz et al., 2004). The patches, which generally last from one to seven days, depending on the drug, have enabled new therapies and reduced first-pass effects and severe side effects. For example, estradiol patches, which are widely used, have eliminated liver damage, which was a side effect of the drug when it was delivered orally. Transdermal clonidine, nitroglycerin, and fentanyl patches also have fewer adverse effects than the same drugs delivered orally. Nicotine patches have prevented, or at least reduced, smoking and increased lifespans (Prausnitz et al., 2004).

Two classes of transdermal patches are currently available: (1) reservoir-type patches and (2) matrix-type patches. A reservoir-type patch holds the drug in a solution or gel, and the rate of delivery is governed by a rate-controlling membrane. Reservoir-type patches offer more flexibility in terms of drug formulation and tighter control over delivery rates than matrix-type patches. However, they are usually associated with greater design complexity. In matrix-type patches, the drug, adhesive, and mechanical backbone of the patch are combined into a single layer. Thus matrix-type patches are easier to fabricate, but they pose even more significant design constraints than reservoir-type patches (Prausnitz et al., 2004).

Drugs that are currently administered transdermally have two common characteristics—low molecular weight and high lipophilicity. Opening the transdermal route to large hydrophilic drugs, a major challenge in the field of transdermal drug delivery, will require the development of technologies that enable the controlled, reproducible transdermal delivery of macromolecular drugs.

Drugs delivered
transdermally have two
common characteristics—
low molecular weight and
high lipophilicity.

Passive Methods
Technologies that facilitate transdermal drug delivery can work either passively or actively, depending on whether an external source of energy is used to facilitate skin permeation (Figure 2). Passive methods include chemical enhancers, micelles, liposomes, and peptides (Chen et al., 2006; El Maghraby et al., 2006; Karande et al., 2004; Schreier and Bouwstra, 1994; Schuetz et al., 2005). Examples of chemical enhancers include fatty acids, fatty esters, solvents, and surfactants (Williams and Barry, 1992). These enhancers facilitate transdermal transport by making drugs more soluble, increasing partitioning into the skin, fluidizing the crystalline structure of the topmost layer of skin, or dissolving skin lipids.

Figure 2 various modes of transdermal drug delivery.

FIGURE 2 Various modes of transdermal drug delivery. (A) Liquid-jet injections deliver drugs into intramuscular, subcutaneous, or intradermal regions. (B) Permeability-based methods of transdermal drug delivery: (i) delivery through hair follicles; (ii) tape-stripping removes the stratum corneum and facilitates drug absorption; (iii) thermal or radio frequency wave-mediated ablation of the stratum corneum creates micropores that enhance drug delivery; (iv) colloidal carriers, such as microemulsions and transfersomes, enhance the dermal absorption of topically applied drugs; (v) low-frequency ultrasound increases drug delivery by making the skin more permeable; (vi) chemical enhancers or peptides for drug delivery; (vii) electroporation of the stratum corneum enhances drug delivery into the epidermis; (viii) microneedles penetrate into the epidermis to deliver drugs. (C) Powder injection delivers dry drug powders into superficial skin layers (epidermis and superficial dermis). Source: Adapted from Mitragotri, 2005.

Although individual chemical enhancers have had some success, combinations of chemical enhancers are more effective. However, so far, the rational design of combinations of enhancers has been limited by the lack of information on interactions between individual chemical enhancers and the stratum corneum. The number of randomly generated formulations for binary mixtures is in the millions, and the number for higher order formulations (for example, ternary or quaternary mixtures) is even higher. Screening of these formulations is beyond the scope of traditional methods (e.g., Franz diffusion cells).

High-throughput methods of screening transdermal formulations can open this bottleneck and may lead to the discovery of previously unknown mixtures. A new high-throughput method for screening transdermal formulations (Karande et al., 2004) is > 100-fold more efficient than Franz diffusion cells (Bronaugh, 1989); with this method, up to 1,000 experiments a day can be conducted, an experimental space well beyond the scope of traditional tools (Karande and Mitragotri, 2001). Recent studies have also shown that peptides may effectively increase skin permeability. Specifically, peptides discovered using phage-display methodology have been shown to deliver macromolecules, such as insulin, in vivo (Chen et al., 2006).

Chemical enhancers are relatively easy to incorporate into transdermal patches and can be calibrated to deliver predetermined amounts of a drug by changing the application area. However, passive methods cannot dynamically control the drug dose.

Active Methods
Active methods can be controlled in real time by varying appropriate parameters. The device and application parameters can also be adjusted to match the patient’s skin properties. A growing number of researchers are now exploring transdermal devices with active mechanisms for skin permeation, such as microneedles, jet injectors, ultrasound, iontophoresis, and electrophoresis (Arora et al., 2007; Bashir et al., 2001; Doukas and Kollias, 2004; Habash et al., 2006; Kalia et al., 2004; Karande et al., 2004; Mitragotri et al., 1995; Prausnitz et al., 1993; Zhang et al., 1996).

Microneedles are arrays of micrometer-sized shallow needles that penetrate only into the superficial layers of skin, thereby eliminating the pain associated with standard hypodermic needles (Prausnitz, 2004). Microneedles have been made from a variety of materials, including metals, semiconductors, polymers, and glass, and have been shown to be effective in drug delivery. They have also been produced in solid and hollow forms. Solid microneedles are used to render skin permeable, whereas hollow microneedles actively deliver drugs into the skin at a controlled rate.

In contrast, jet injectors deliver a high-velocity liquid jet stream into the skin, delivering drugs into various skin layers, depending on the jet parameters (Mitragotri, 2006). Jet injectors have a long history, particularly in the delivery of vaccines, insulin, and growth hormone. Ultrasound enhances skin permeability by cavitation, which temporarily disrupts skin structure (Paliwal et al., 2006; Tezel and Mitragotri, 2003). Iontophoresis and electroporation use electric fields to alter skin structure and/or provide additional driving force for drug penetration through the skin (Banga and Prausnitz, 1998; Guy et al., 2000).

Combined Technologies
Although many individual technologies have been shown to facilitate transderml drug transport, combinations of technologies are often more effective than any of them alone (Mitragotri, 2000). A combination of two or more technologies may not only increase the enhancement, but may also potentially be safer. Understanding the synergies between technologies and selecting the right combinations is a fruitful area for research that is still largely unexplored.

In the last decade, significant new insights have been developed into the structural organization and barrier formation of the skin. In the past, skin was considered primarily a barrier, but it is now known to be a smart material that controls its own structure and function in response to the environment (Menon, 2002). This new knowledge must be incorporated into the future development and evaluation of transdermal technologies.

Oral Drug Delivery
Oral drug delivery is the most common, and the preferred type of drug administration. A large number of small molecules, including those prescribed for the treatment of pain, heart disease, and blood pressure, are already delivered orally. Drugs delivered orally are typically absorbed across the intestinal epithelium into the bloodstream via two mechanisms. The trans-cellular route involves the transport of drugs through the cell membrane to cross the barrier, either by partitioning of the drug into cell membranes or through the generation of small pores in the outer cell membrane, which allows entry into the cell.

Alternatively, the drug may permeate through the paracellular pathway, which entails transport through the tight junctions between epithelial cells (Cano-Cebrian et al., 2005). A tight junction is a dynamic network of tightly packed proteins in the interstitial spaces of a cell monolayer. Tight junctions have been likened to gatekeepers, as their primary function is to maintain the barrier properties of the epithelium and only permit the transport of very small molecules (< 4 nm in diameter).

A third possibility is that drugs may be actively transported across the epithelium through receptor-mediated endocytosis (Figure 3).

Figure 3 Pathways of drug absorption across the intestinal epithelium.

FIGURE 3 Pathways of drug absorption across the intestinal epithelium. Source: Adapted from Mitragotri, 2005.

Proteins and Peptides
The oral delivery of proteins and peptides has elicited a great deal of interest in recent years because of the availability of novel therapeutics through the advent of recombinant DNA technology. Proteins and peptides are macromolecules with a wide variety of functions in biological catalysis, the regulation of cellular processes, and immune-system protection.

Effective oral delivery of a protein or peptide requires that a therapeutic molecule be delivered to the site of interest and cross the intestinal epithelium barrier intact before being transported to the portal circulation system. Unfortunately, this process is difficult and results in only a small fraction of drug being absorbed in the bloodstream. The delivery of proteins and peptides is further limited by their susceptibility to enzymatic degradation in the gastrointestinal tract (Morishita and Peppas, 2006).

The scientific community has made a major effort in recent years to overcome the obstacles to oral delivery through the development of a large number of new, innovative drug-delivery techniques (Hosny et al., 2002; Luessen et al., 1995; Lyu et al., 2004; Sinha et al., 2004; Whitehead and Mitragotri, 2008; Whitehead et al., 2004, 2008a;b). These methods include enzyme inhibitors, permeation enhancers, mucoadhesive polymers, chemical modification of drugs, targeted delivery, and encapsulation.

With engineering tools
at hand, the future of
drug delivery looks
brighter than ever.

Enzyme Inhibitors
Enzyme inhibitors are used to counteract the natural functions of the enzymes of the gastrointestinal tract that break down ingested proteins. Many studies have been performed in which inhibitors were co-administered with a drug (Bernkop-Schnurch, 1998), but these strategies have seldom been successful unless they included absorption enhancers.

Permeation enhancers have also been used, similar to the way they are used in transdermal drug delivery (Carino and Mathiowitz, 1999). Permeation enhancers, such as surfactants, fatty acids, and bile salts, either disrupt the epithelial membrane of the intestine or loosen the tight junctions between epithelial cells. While numerous studies have demonstrated that certain enhancers can be very potent delivery aids, safety concerns abound (Aungst, 2000).

Mucoadhesive strategies have also been used to localize drugs to a small, defined region of the intestine through attractive interactions between the carrier and the intestinal epithelium. This kind of localization results in a high concentration gradient of the drug across the epithelial barrier, which improves drug bioavailability. In addition, a strong adhesion force prolongs the residence time of the dosage at the site of drug absorption, which reduces the dosing frequency and, in turn, increases patient compliance.

Certain mucoadhesive polymers, such as polycarbophil and chitosan derivatives, have been shown to simultaneously act as permeation enhancers and enzyme inhibitors (Luessen et al., 1995; Sinha et al., 2004).

Encapsulation Technologies
Encapsulation technologies are another alternative for the oral administration of drugs. Using commercially available pH-sensitive polymers, it is possible to target particular regions of the intestine (e.g., jejunum or colon) for drug delivery. Enteric coatings made from these pH-sensitive polymers enable drug-delivery devices to pass through the acidic environment of the stomach unscathed and rapidly dissolve in the intestine. Studies to evaluate these polymers for targeted oral delivery are ongoing in various laboratories (Hosny et al., 2002; Lyu et al., 2004).

Other techniques involve the targeting of M-cells in the intestine to improve mucosal vaccine delivery. M-cells, which are present in the Peyer’s patches of the intestine, have the unique ability to take up antigens; targeting can be achieved by using M-cell-specific lectins in combination with a drug-delivery formulation.

Other encapsulation strategies, including micro-particles (Mathiowitz et al., 1997), nanoparticles (Carino et al., 2000), and liposomes (Iwanaga et al., 1999), have been developed. These strategies can protect proteins from enzymatic degradation in the intestine and/or facilitate protein uptake across the epithelium (Carino and Mathiowitz, 1999).

Areas for Ongoing Research
Novel, painless, patient-friendly methods of drug delivery represent an unmet need in the field of health care. Discoveries in the last decade have demonstrated the feasibility of using several different methodologies for enhancing drug delivery through skin and other mucosal surfaces. These methods have shown the potential to deliver several molecules, including macro-molecules such as insulin and vaccines.

The development of mathematical models to describe and predict transport across the skin and mucosal barriers is another area of active research that has provided useful insights into the development of novel strategies. With the variety of engineering tools at hand, the future of drug delivery looks brighter than ever. The challenge is to convert these discoveries into useful products.

Arora, A., I. Hakim, J. Baxter, R. Rathnasingham, R. Srinivasan, D.A. Fletcher, and S. Mitragotri. 2007. Needle-free delivery of macromolecules across the skin by nanoliter-volume pulsed microjets. Proceedings of the National Academy of Sciences of the United States of America 104(11): 4255–4260.

Aungst, B. 2000. Intestinal permeation enhancers. Journal of Pharmacological Science 89(4): 429–442.

Banga, A.K., and M.R. Prausnitz. 1998. Assessing the potential of skin electroporation for the delivery of protein- and gene-based drugs. Trends in Biotechnology 16(10): 408–412.

Bashir, S.L., A.L. Chew, A. Anigbogu, F. Dreher, and H.I. Maibach. 2001. Physical and physiological effects of stratum corneum tape stripping. Skin Research Technology 7(1): 40–48.

Bernkop-Schnurch, A. 1998. The use of inhibitory agents to overcome the enzymatic barrier to perorally administered therapeutic peptides and proteins. Journal of Controlled Release 52(1-2): 1–16.

Bronaugh, R.L. 1989. Determination of Percutaneous Absorption by In Vitro Techniques. Pp. 239–259 in Percutaneous Absorption: Mechanisms, Methodology, and Drug Delivery, edited by R.L. Bronaugh and H.I. Maibach. New York and Basel: Marcel Dekker Inc.

Cano-Cebrian, M.J., T. Zornoza, L. Granero, and A. Polache. 2005. Intestinal absorption enhancement via the paracellular route by fatty acids, chitosans and others: a target for drug delivery. Current Drug Delivery 2(1): 9–22.

Carino, G.P., and E. Mathiowitz. 1999. Oral insulin delivery. Advanced Drug Delivery Reviews 35(2-3): 249–257.

Carino, G.P, J.S. Jacob, and E. Mathiowitz. 2000. Nanosphere based oral insulin delivery. Journal of Controlled Release 65(1-2): 261–269.

Chen, Y., Y. Shen, X. Guo, C. Zhang, W. Yang, M. Ma, S. Liu, M. Zhang, and L.P. Wen. 2006. Transdermal protein delivery by a coadministered peptide identified via phage display. Nature Biotechnology 24(4): 455–460.

Doukas, A.G., and N. Kollias. 2004. Transdermal drug delivery with a pressure wave. Advanced Drug Delivery Reviews 56(5): 559–579.

El Maghraby, G.M., A.C. Williams, and B.W. Barry. 2006. Can drug-bearing liposomes penetrate intact skin? Journal of Pharmacy and Pharmacology 58(4): 415–429.

Guy, R.H., Y.N. Kalia, M.B. Delgado-Charro, V. Merino, A. López, and D. Marro. 2000. Iontophoresis: electrorepulsion and electroosmosis. Journal of Controlled Release 64(1-3): 129–132.

Habash, R.W.Y., R. Bansal, D. Krewski, and H.I. Alhafid. 2006.   Thermal therapy, part 1: an introduction to thermal therapy. Critical Reviews in Biomedical Engineering 34(6): 459–489.

Hosny, E.A., H.I. Al-Shora, and M.M. Elmazar. 2002. Oral delivery of insulin from enteric-coated capsules containing sodium salicylate: effect on relative hypoglycemia of diabetic beagle dogs. International Journal of Pharmacology 237(1-2): 71–76.

Iwanaga, K., S. Ono, K. Narioka, M. Kakemi, K. Morimoto, S. Yamashita, Y. Namba, and N. Oku. 1999. Application of surface-coated liposomes for oral delivery of peptide: effects of coating the liposome’s surface on the GI transit of insulin. Journal of Pharmacological Sciences 88(2): 248–252.

Kalia, Y.N., A. Naik, J. Garrison, and R.H. Guy. 2004. Iontophoretic drug delivery. Advanced Drug Delivery Reviews 56(5): 619–658.

Kane, A., J. Lloyd, M. Zaffran, L. Simonsen, and M. Kane. 1999. Transmission of hepatitis B, hepatitis C and human immunodeficiency viruses through unsafe injections in the developing world: model-based regional estimates. Bulletin of the World Health Organization 77(10): 801–807.

Karande, P., and S. Mitragotri. 2001. High throughput screening of transdermal formulations. Pharmaceutical Research 19(5): 655–660.

Karande, P., A. Jain, and S. Mitragotri. 2004. Discovery of transdermal penetration enhancers by high-throughput screening. Nature Biotechnology 22(2): 192–197.

Kermode, M. 2004. Unsafe injections in low-income country health settings: need for injection safety promotion to prevent the spread of blood-borne viruses. Health Promotion International 19(1): 95–103.

Langer, R. 2003. Where a pill won’t reach. Scientific American 288(4): 50–57.

Luessen, H.I., J.C. Verhoef, G. Borchard, C.M. Lehr, A.G. deBoer, H.E. Junginger. 1995. Mucoadhesive polymers in peroral peptide drug delivery. II. Carbomer and polycarbophil are potent inhibitors of the intestinal proteolytic enzyme trypsin. Pharmaceutical Research 12(9): 1293–1298.

Lyu, S.Y., Y.J. Kwon, H.J. Joo, and W.B. Park. 2004. Preparation of alginate/chitosan microcapsules and enteric coated granules of mistletoe lectin. Archives of Pharmacal Research 27(1): 118–126.

Mathiowitz, E., J.S. Jacob, Y.S. Jong, G.P. Carino, D.E. Chickering, P. Chaturvedi, C.A. Santos, K. Vijayaraghavan, S. Montgomery, M. Bassett, and C. Morrell. 1997. Biologically erodable microspheres as potential oral drug delivery systems. Nature 386(6623): 410–414.

Menon, G. K. 2002. New insights into skin structure: scratching the surface. Advanced Drug Delivery Reviews 54(Suppl. 1): S3–S17.

Mitragotri, S. 2000. Synergistic effect of enhancers for transdermal drug delivery. Pharmaceutical Research 17(11): 1354–1359.

Mitragotri, S. 2005. Immunization without needles. Nature Reviews. Immunology 5(12): 905–916.

Mitragotri, S. 2006. Current status and future prospects of needle-free liquid jet injectors. Nature Reviews. Drug Discovery 5(7): 543–548.

Mitragotri, S., D. Blankschtein, and R. Langer. 1995. Ultrasound-mediated transdermal protein delivery. Science 269(5255): 850–853.

Morishita, M., and N.A. Peppas. 2006. Is the oral route possible for peptide and protein drug delivery? Drug Discovery Today 11(19-20): 905–910.

Nir, Y., A. Paz, E. Sabo, and J. Potasman. 2003. Fear of injections in young adults: prevalence and associations. American Journal of Tropical Medicine and Hygiene 68(3): 341–344.

Paliwal, S., G.K. Menon, and S. Mitragotri. 2006. Low-frequency sonophoresis: ultrastructural basis for stratum corneum permeability assessed using quantum dots. Journal of Investigative Dermatology 126(5): 1095–1101.

Prausnitz, M.R. 2004. Microneedles for transdermal drug delivery. Advanced Drug Delivery Reviews 56(5): 581–587.

Prausnitz, M.R., V.G. Bose, R. Langer, and J.C. Weaver. 1993. Electroporation of mammalian skin: a mechanism to enhance transdermal drug delivery. Proceedings of the National Academy of Sciences of the United States of America 90(22): 10504–10508.

Prausnitz, M.R., S. Mitragotri, and R. Langer. 2004. Current status and future potential of transdermal drug delivery. Nature Reviews. Drug Discovery 3(2): 115–124.

Rosenstock, L. 2000. Needlestick Injuries Among Healthcare Workers. Statement of the Director, CDC National Institute for Occupational Safety and Health, before the House Subcommittee on Workforce Protections, Committee on Education and the Workforce, U.S. House of Representatives, June 22, 2000.

Schreier, H., and J. Bouwstra. 1994. Liposomes and niosomes as topical drug carriers: dermal and transdermal drug delivery. Journal of Controlled Release 30(1): 1–15.

Schuetz, Y.B., A. Naik, R.H. Guy, and Y.N. Kalia. 2005. Emerging strategies for the transdermal delivery of peptide and protein drugs. Expert Opinion on Drug Delivery 2(3): 533–548.

Sinha, V.R., A.K. Singla, S. Wadhawan, R. Kaushik, R. Kumria, K. Bansal, and S. Dhawan. 2004. Chitosan microspheres as a potential carrier for drugs. International Journal of Pharmaceutics 274(1-2): 1–33.

Tezel, A., and S. Mitragotri. 2003. Interactions of inertial cavitation bubbles with stratum corneum lipid bilayers during low-frequency sonophoresis. Biophysical Journal 85(6): 3502–3512.

Varmus, H., R. Klausner, E. Zerhouni, T. Acharya, A.S. Daar, and P.A. Singer. 2003. Public health. grand challenges in global health. Science 302(5644): 398–399.

Vasir, J.K., and V. Labhasetwar. 2005. Targeted drug delivery in cancer therapy. Technology in Cancer Research & Treatment 4(4): 363–374.

Wertz, P.W., and D.T. Downing. 1989. Stratum Corneum: Biological and Biochemical Considerations. Pp. 1–17 in Transdermal Drug Delivery: Developmental Issues and Research Initiatives, edited by J. Hadgraft and R.H. Guy. New York and Basel: Marcel Dekker Inc.

Whitehead, K., and S. Mitragotri. 2008. Mechanistic analysis of chemical permeation enhancers for oral drug delivery. Pharmaceutical Research 25(6): 1412–1419.

Whitehead, K., Z. Shen, and S. Mitragotri. 2004. Oral delivery of macromolecules using intestinal patches: applications for insulin delivery. Journal of Controlled Release 98(1): 37–45.

Whitehead, K., N. Karr, and S. Mitragotri. 2008a. Discovery of synergistic permeation enhancers for oral drug delivery. Journal of Controlled Release 128(2): 128–133.

Whitehead, K., N. Karr, and S. Mitragotri. 2008b. Safe and effective permeation enhancers for oral drug delivery. Pharmeutical Research 25(8): 1782–1788.

Williams, A.C., and B.W. Barry. 1992. Skin absorption enhancers. Critical Reviews in Therapeutic Drug Carrier Systems 9(3-4): 305–253.

Zaffaroni, A. 1991. Overview and evolution of therapeutic systems. Annals of the New York Academy of Sciences 618: 405–421.

Zhang, I., K.K. Shung, and D.A. Edwards. 1996. Hydrogels with enhanced mass transfer for transdermal drug delivery. Journal of Pharmaceutical Science 85(12): 1312–1316.



About the Author:Samir Mitragotri is a professor of chemical engineering at the University of California, Santa Barbara.